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Sialic acid: a preventable signal for pneumococcal biofilm formation, colonization, and invasion of the host.

The Journal of infectious diseases (2009-04-28)
Claudia Trappetti, Aras Kadioglu, Melissa Carter, Jasvinder Hayre, Francesco Iannelli, Gianni Pozzi, Peter W Andrew, Marco R Oggioni
ABSTRACT

The correlation between carbohydrate availability, pneumococcal biofilm formation, nasopharyngeal colonization, and invasion of the host has been investigated. Of a series of sugars, only sialic acid (i.e., N-acetylneuraminic acid) enhanced pneumococcal biofilm formation in vitro, at concentrations similar to those of free sialic acid in human saliva. In a murine model of pneumococcal carriage, intranasal inoculation of sialic acid significantly increased pneumococcal counts in the nasopharynx and instigated translocation of pneumococci to the lungs. Competition of both sialic acid-dependent phenotypes was found to be successful when evaluated using the neuraminidase inhibitors DANA (i.e., 2,3-didehydro-2-deoxy-N-acetylneuraminic acid), zanamivir, and oseltamivir. The association between levels of free sialic acid on mucosae, pneumococcal colonization, and development of invasive disease shows how a host-derived molecule can influence a colonizing microbe and also highlights a molecular mechanism that explains the epidemiologic correlation between respiratory infections due to neuraminidase-bearing viruses and bacterial pneumonia. The data provide a new paradigm for the role of a host compound in infectious diseases and point to new treatment strategies.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Rhamnose monohydrate, ≥99%
Sigma-Aldrich
D-(+)-Xylose, ≥99%
Sigma-Aldrich
N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, ≥93%