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  • A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.

A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.

Molecular bioSystems (2010-03-20)
Ying Huang, William K Russell, Wei Wan, Pei-Jing Pai, David H Russell, Wenshe Liu
ABSTRACT

By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nε-Acetyl-L-lysine