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Cometabolic degradation of trichloroethene by Rhodococcus sp. strain L4 immobilized on plant materials rich in essential oils.

Applied and environmental microbiology (2010-05-18)
Oramas Suttinun, Rudolf Müller, Ekawan Luepromchai
ABSTRACT

The cometabolic degradation of trichloroethene (TCE) by Rhodococcus sp. L4 was limited by the loss of enzyme activity during TCE transformation. This problem was overcome by repeated addition of inducing substrates, such as cumene, limonene, or cumin aldehyde, to the cells. Alternatively, Rhodococcus sp. L4 was immobilized on plant materials which contain those inducers in their essential oils. Cumin seeds were the most suitable immobilizing material, and the immobilized cells tolerated up to 68 muM TCE and degraded TCE continuously. The activity of immobilized cells, which had been inactivated partially during TCE degradation, could be reactivated by incubation in mineral salts medium without TCE. These findings demonstrate that immobilization of Rhodococcus sp. L4 on plant materials rich in essential oils is a promising method for efficient cometabolic degradation of TCE.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Cumene, 98%
Sigma-Aldrich
Cuminaldehyde, 98%
Supelco
Cumene, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Cuminaldehyde, ≥98%, FCC, FG
Supelco
Cumene, PESTANAL®
Supelco
Cumene solution, certified reference material, 5000 μg/mL in methanol