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EOBII, a gene encoding a flower-specific regulator of phenylpropanoid volatiles' biosynthesis in petunia.

The Plant cell (2010-06-15)
Ben Spitzer-Rimon, Elena Marhevka, Oren Barkai, Ira Marton, Orit Edelbaum, Tania Masci, Naveen-Kumar Prathapani, Elena Shklarman, Marianna Ovadis, Alexander Vainstein
ABSTRACT

Floral scent, which is determined by a complex mixture of low molecular weight volatile molecules, plays a major role in the plant's life cycle. Phenylpropanoid volatiles are the main determinants of floral scent in petunia (Petunia hybrida). A screen using virus-induced gene silencing for regulators of scent production in petunia flowers yielded a novel R2R3-MYB-like regulatory factor of phenylpropanoid volatile biosynthesis, EMISSION OF BENZENOIDS II (EOBII). This factor was localized to the nucleus and its expression was found to be flower specific and temporally and spatially associated with scent production/emission. Suppression of EOBII expression led to significant reduction in the levels of volatiles accumulating in and emitted by flowers, such as benzaldehyde, phenylethyl alcohol, benzylbenzoate, and isoeugenol. Up/downregulation of EOBII affected transcript levels of several biosynthetic floral scent-related genes encoding enzymes from the phenylpropanoid pathway that are directly involved in the production of these volatiles and enzymes from the shikimate pathway that determine substrate availability. Due to its coordinated wide-ranging effect on the production of floral volatiles, and its lack of effect on anthocyanin production, a central regulatory role is proposed for EOBII in the biosynthesis of phenylpropanoid volatiles.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Benzyl benzoate, ReagentPlus®, ≥99.0%
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Benzyl benzoate, natural, ≥99%, FCC, FG
Supelco
Benzyl benzoate, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Benzyl benzoate, analytical standard
Sigma-Aldrich
Benzyl benzoate, ≥99%, FCC, FG
Sigma-Aldrich
Benzyl benzoate, meets USP testing specifications
Sigma-Aldrich
Benzyl benzoate, tested according to Ph. Eur.