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Dynamics of water soluble phosphorus from surface applied broiler litter.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes (2010-07-28)
Armando S Tasistro, Liliana Picone, Miguel Cabrera
ABSTRACT

Deionized water is routinely used as an extractant to determine soluble phosphorus (P) in broiler litter, but under N.E. Georgia conditions this technique may underestimate the hazard of P loss in runoff because the alkalinity of the broiler litter-water suspension limits the solubility of P compounds that may be solubilized after being spread on acidic field conditions. In this study under controlled conditions we measured soluble P in thatch and top soil after applying untreated broiler litter, residue of broiler litter after water extraction (WER), or residue of broiler litter after extraction with a 2-(N-morpholino) ethanesulfonic acid (MES) buffer at pH 6 (BER). During the 60 d incubation, the WER released 18% more Total Dissolved P (TDP) than was determined through a conventional water extraction procedure, whereas the BER released 28% less TDP than the WER, which reflects the greater amount of TDP removed from the broiler litter by the buffer at pH 6.0. However, the total amount of TDP extracted by the MES buffer, which includes that removed at the initial extraction plus that released during the incubation, was 30% greater than the total amount of TDP extracted with water from the untreated litter plus the TDP extracted with water from the WER during the incubation. This result suggests the need to fine-tune the solid: liquid ratio and shaking time when the MES buffer is used.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MES hydrate, ≥99.5% (titration)
Sigma-Aldrich
MES, low moisture content, ≥99% (titration)
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MES hydrate, BioPerformance Certified, suitable for cell culture, ≥99.5%
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MES hydrate, ≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra
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MES sodium salt, ≥99% (titration)
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MES sodium salt, BioReagent, ≥99% (titration), crystalline
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MES solution, BioUltra, for molecular biology, 0.5 M in H2O
Sigma-Aldrich
MES sodium salt, BioPerformance Certified, suitable for cell culture
SAFC
MES sodium salt
Sigma-Aldrich
MES hydrate, BioUltra, ≥99.5% (T)