Deionized water is routinely used as an extractant to determine soluble phosphorus (P) in broiler litter, but under N.E. Georgia conditions this technique may underestimate the hazard of P loss in runoff because the alkalinity of the broiler litter-water suspension limits the solubility of P compounds that may be solubilized after being spread on acidic field conditions. In this study under controlled conditions we measured soluble P in thatch and top soil after applying untreated broiler litter, residue of broiler litter after water extraction (WER), or residue of broiler litter after extraction with a 2-(N-morpholino) ethanesulfonic acid (MES) buffer at pH 6 (BER). During the 60 d incubation, the WER released 18% more Total Dissolved P (TDP) than was determined through a conventional water extraction procedure, whereas the BER released 28% less TDP than the WER, which reflects the greater amount of TDP removed from the broiler litter by the buffer at pH 6.0. However, the total amount of TDP extracted by the MES buffer, which includes that removed at the initial extraction plus that released during the incubation, was 30% greater than the total amount of TDP extracted with water from the untreated litter plus the TDP extracted with water from the WER during the incubation. This result suggests the need to fine-tune the solid: liquid ratio and shaking time when the MES buffer is used.