The supercritical fluid chromatography of intact aliphatic amines with different columns is described. One group of amines was based on N,N-dimethyl-n-octylamine and related primary and secondary amines, and the other on the amino alcohol metoprolol and several of its analogues. Columns with three different phases were investigated, one non-polar coated with 5% phenyl methyl polysiloxane and two more polar with 25% cyanopropyl methylphenyl polysiloxane and Carbowax 20M. Generally, equal molar amounts were injected under splitless conditions and the peak symmetry was recorded. The system with the non-polar silicone phase was more inert, followed by the wax-phase column. The cyanopropyl column gave severe peak tailing although it was loaded with five times more of the amines than the other columns. The selectivity was investigated and was found higher with the two polar columns. Both showed a marked increase in the retention of amines with free hydrogens. With nitrous oxide the selectivity was almost the same as that with carbon dioxide as mobile phase. The nature of the flame ionization detector changed, however, giving a negative baseline drift on pressure programming. An interesting conclusion is that the amines are chromatographed as such with carbon dioxide as the mobile phase.
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