MilliporeSigma
  • Home
  • Search Results
  • Food additives such as sodium sulphite, sodium benzoate and curcumin inhibit leptin release in lipopolysaccharide-treated murine adipocytes in vitro.

Food additives such as sodium sulphite, sodium benzoate and curcumin inhibit leptin release in lipopolysaccharide-treated murine adipocytes in vitro.

The British journal of nutrition (2011-08-02)
Christian Ciardi, Marcel Jenny, Alexander Tschoner, Florian Ueberall, Josef Patsch, Michael Pedrini, Christoph Ebenbichler, Dietmar Fuchs
ABSTRACT

Obesity leads to the activation of pro-inflammatory pathways, resulting in a state of low-grade inflammation. Recently, several studies have shown that the exposure to lipopolysaccharide (LPS) could initiate and maintain a chronic state of low-grade inflammation in obese people. As the daily intake of food additives has increased substantially, the aim of the present study was to investigate a potential influence of food additives on the release of leptin, IL-6 and nitrite in the presence of LPS in murine adipocytes. Leptin, IL-6 and nitrite concentrations were analysed in the supernatants of murine 3T3-L1 adipocytes after co-incubation with LPS and the food preservatives, sodium sulphite (SS), sodium benzoate (SB) and the spice and colourant, curcumin, for 24 h. In addition, the kinetics of leptin secretion was analysed. A significant and dose-dependent decrease in leptin was observed after incubating the cells with SB and curcumin for 12 and 24 h, whereas SS decreased leptin concentrations after 24 h of treatment. Moreover, SS increased, while curcumin decreased LPS-stimulated secretion of IL-6, whereas SB had no such effect. None of the compounds that were investigated influenced nitrite production. The food additives SS, SB and curcumin affect the leptin release after co-incubation with LPS from cultured adipocytes in a dose- and time-dependent manner. Decreased leptin release during the consumption of nutrition-derived food additives could decrease the amount of circulating leptin to which the central nervous system is exposed and may therefore contribute to an obesogenic environment.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sodium benzoate, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Sodium benzoate, ReagentPlus®, 99%
Sigma-Aldrich
Sodium benzoate, purum p.a., ≥99.0% (NT)
Sigma-Aldrich
Sodium benzoate, BioXtra, ≥99.5%
Sigma-Aldrich
Sodium benzoate, puriss., meets analytical specification of Ph. Eur., BP, FCC, E211, 99.0-100.5% (calc. to the dried substance), powder
Sigma-Aldrich
Sodium sulfite, BioUltra, anhydrous, ≥98% (RT)
Sigma-Aldrich
Sodium sulfite, BioXtra, ≥98%
Sigma-Aldrich
Sodium sulfite, anhydrous, Redi-Dri, reagent grade, ≥98%
Sigma-Aldrich
Sodium sulfite, ≥98%
Sigma-Aldrich
Sodium sulfite, puriss., meets analytical specification of Ph. Eur., BP, NF, anhydrous, 95.0-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, puriss. p.a., reag. Ph. Eur., anhydrous, 98-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, ACS reagent, ≥98.0%