• Home
  • Search Results
  • Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13.

Bioproduction of vanillin using an organic solvent-tolerant Brevibacillus agri 13.

Applied microbiology and biotechnology (2011-08-05)
Nuttawat Wangrangsimagul, Kunticha Klinsakul, Alisa S Vangnai, Jirarut Wongkongkatep, Pranee Inprakhon, Kohsuke Honda, Hisao Ohtake, Junichi Kato, Thunyarat Pongtharangkul
ABSTRACT

Nowadays, majority of vanillin supplied to the world market is chemically synthesized from a petroleum-based raw material, raising a concern among the consumers regarding the product safety. In this study, an organic solvent-tolerant Brevibacillus agri 13 previously reported for a strong predilectic property was utilized as a whole-cell biocatalyst for bioproduction of vanillin from isoeugenol (IG). B. agri 13 is the first biocatalyst reported for bioproduction of vanillin at a temperature as high as 45°C. Both pH and temperature were found to affect vanillin production significantly. An extreme level of organic solvent tolerance of B. agri 13 allowed us to utilize it in a biphasic system using organic solvents generally considered as highly toxic to most bacteria. With an addition of butyl acetate at 30% (v/v) as an organic second phase, toxicity of IG exerted onto the biocatalyst was reduced dramatically while faster and more efficient vanillin production was obtained (1.7 g/L after 48 h with 27.8% molar conversion).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Butyl acetate, anhydrous, ≥99%
Sigma-Aldrich
Butyl acetate, ACS reagent, ≥99.5%
Sigma-Aldrich
Butyl acetate, suitable for HPLC, 99.7%
Sigma-Aldrich
Butyl acetate, natural, ≥98%, FG
Supelco
Butyl acetate, analytical standard
Sigma-Aldrich
Butyl acetate, ≥99%, FCC, FG
Sigma-Aldrich
Isoeugenol, 98%, mixture of cis and trans
Sigma-Aldrich
Isoeugenol, mixture of cis and trans, 99%, FG
Sigma-Aldrich
Butyl acetate, ReagentPlus®, 99.5%
Supelco
Isoeugenol, analytical standard
Sigma-Aldrich
Butyl acetate, puriss. p.a., ACS reagent
Supelco
Butyl acetate, Pharmaceutical Secondary Standard; Certified Reference Material