• Home
  • Search Results
  • Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.

Improved tumor targeting of polymer-based nanovesicles using polymer-lipid blends.

Bioconjugate chemistry (2011-09-09)
Zhiliang Cheng, Drew R Elias, Neha P Kamat, Eric D Johnston, Andrei Poloukhtine, Vladimir Popik, Daniel A Hammer, Andrew Tsourkas
ABSTRACT

Block copolymer-based vesicles have recently garnered a great deal of interest as nanoplatforms for drug delivery and molecular imaging applications due to their unique structural properties. These nanovesicles have been shown to direct their cargo to disease sites either through enhanced permeability and retention or even more efficiently via active targeting. Here, we show that the efficacy of nanovesicle targeting can be significantly improved when prepared from polymer-lipid blends compared with block copolymer alone. Polymer-lipid hybrid nanovesicles were produced from the aqueous coassembly of the diblock copolymer, poly(ethylene oxide)-block-polybutadiene (PEO-PBD), and the phospholipid, hydrogenated soy phosphatidylcholine (HSPC). The PEG-based vesicles, 117 nm in diameter, were functionalized with either folic acid or anti-HER2/neu affibodies as targeting ligands to confer specificity for cancer cells. Our results revealed that nanovesicles prepared from polymer-lipid blends led to significant improvement in cell binding compared to nanovesicles prepared from block copolymer alone in both in vitro cell studies and murine tumor models. Therefore, it is envisioned that nanovesicles composed of polymer-lipid blends may constitute a preferred embodiment for targeted drug delivery and molecular imaging applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Polybutadiene, average Mw ~200,000
Sigma-Aldrich
Polybutadiene, predominantly 1,2-addition, approx. 90% 1,2-vinyl
Sigma-Aldrich
Polybutadiene, average Mn ~5,000
Sigma-Aldrich
Polybutadiene, cis, average Mw 200,000-300,000
Sigma-Aldrich
Polybutadiene, average Mn 1,530-2,070 by VPO
Sigma-Aldrich
Atto 740 maleimide, suitable for fluorescence
Sigma-Aldrich
Atto 740-Biotin, suitable for fluorescence, ≥90.0% (HPCE)
Supelco
Atto 740 NHS ester, BioReagent, suitable for fluorescence, ≥90.0% (coupling to amines)

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.