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Multiwalled carbon nanotubes dispersed in carminic acid for the development of catalase based biosensor for selective amperometric determination of H(2)O(2) and iodate.

Biosensors & bioelectronics (2011-09-09)
Arun Prakash Periasamy, Ya-Hui Ho, Shen-Ming Chen
ABSTRACT

We report the preparation of stable dispersion of multiwalled carbon nanotubes (MWCNTs) using carminic acid (CA) as a dispersing agent. The transmission electron microscopy (TEM), scanning electron microscopy (SEM), and atomic force microscopy (AFM) results confirmed that MWCNT is well dispersed in CA aqueous solution and CA has been well adsorbed at MWCNT walls. Fourier transform infrared (FTIR) and UV-vis absorption spectra results also confirmed the adsorption of CA at MWCNT. To develop a highly selective amperometric biosensor for H(2)O(2) and iodate, the model enzyme catalase (CAT) was immobilized at CACNT modified glassy carbon electrode surface. The immobilized CAT exhibits well defined quasi reversible redox peaks at a formal potential (E°') of -0.559V in 0.05M pH 7 phosphate buffer solution (PBS). The proposed CAT/CACNT biosensor exhibits excellent amperometric response towards H(2)O(2) and iodate in the linear concentration range between 10μM to 3.2mM and 0.01-2.16mM. The sensitivity values are 287.98μAmM(-1)cm(-2) and 0.253mAmM(-1)cm(-2), respectively. Moreover, the developed CAT biosensor exhibits high affinity for H(2)O(2) and iodate with good selectivity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Magnesium iodate tetrahydrate, 99.0%
Supelco
Carminic acid, analytical standard
Sigma-Aldrich
Carminic acid
Sigma-Aldrich
Lithium iodate, 97%
Sigma-Aldrich
Iodic acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Iodic acid, puriss. p.a., ACS reagent, ≥99.5% (RT)