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Bioresolution of benzyl glycidyl ether using whole cells of Bacillus alcalophilus.

Journal of basic microbiology (2011-11-05)
Neeraj Bala, Kirandeep Kaur, Swapandeep Singh Chimni, Harvinder Singh Saini, Shamsher Singh Kanwar
ABSTRACT

The incubation of whole Bacillus alcalophilus cells grown on a mineral supplemented medium (MSM) containing 1% (w/v) sucrose as carbon source, 1.2% (w/v) tryptone as nitrogen source at pH 6.5 and temperature 30 °C in 24 h kinetically resolved benzyl glycidyl ether (1 mg/ml) to provide (S)-benzyl glycidyl ether with 30% ee and (R)-3-benzyloxypropane-1,2-diol with 40% ee.

MATERIALS
Product Number
Brand
Product Description

Millipore
Peptone from milk solids, Refined hydrolysate
Sigma-Aldrich
Peptone from animal tissue, from meat, BioReagent, suitable for cell culture, suitable for plant cell culture
Millipore
Proteose Peptone, Enzymatic hydrolysate
Sigma-Aldrich
Benzyl glycidyl ether, 99%
Millipore
Peptone from casein and other animal proteins, suitable for microbiology
Millipore
Peptone Primatone® RL, suitable for microbiology
Millipore
Peptone from animal tissue, from meat
Sigma-Aldrich
Peptone from animal tissue, BioReagent, Type I, suitable for plant cell culture, from meat
Millipore
Peptone Primatone® RLT, suitable for microbiology