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Extractive fermentation for improved production and recovery of lipase derived from Burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems.

Bioresource technology (2011-11-09)
Pau Loke Show, Chin Ping Tan, Mohd Shamsul Anuar, Arbakariya Ariff, Yus Aniza Yusof, Soo Kien Chen, Tau Chuan Ling
ABSTRACT

An extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular Burkholderia cepacia lipase. A 10% (w/w) solution of ethylene oxide-propylene oxide (EOPO) with a molecular mass of 3900 g/mol and pH 8.5, a 200 rpm speed, and 30 °C were selected as the optimal conditions for lipase production (55 U/ml). Repetitive batch fermentation was performed by continuous replacement of the top phase every 24h, which resulted in an average cell growth mass of 4.7 g/L for 10 extractive batches over 240 h. In scaling-up the process, a bench-scale bioreactor was tested under the conditions that had been optimized in flasks. The production rate and recovery yield were higher in the bioreactor compared to fermentation performed in flasks.

MATERIALS
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Product Description

Sigma-Aldrich
Poly(ethylene glycol-ran-propylene glycol) monobutyl ether, average Mn ~3,900