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  • Microwave-assisted purge-and-trap extraction device coupled with gas chromatography and mass spectrometry for the determination of five predominant odors in sediment, fish tissues, and algal cells.

Microwave-assisted purge-and-trap extraction device coupled with gas chromatography and mass spectrometry for the determination of five predominant odors in sediment, fish tissues, and algal cells.

Journal of chromatography. A (2011-12-14)
Xuwei Deng, Ping Xie, Min Qi, Gaodao Liang, Jun Chen, Zhimei Ma, Yan Jiang
ABSTRACT

Off-flavors are among the most troublesome compounds in the environment worldwide. The lack of a viable theory for studying the sources, distribution, and effect of odors has necessitated the accurate measurement of odors from environmental compartments. A rapid and flexible microwave-assisted purge-and-trap extraction device for simultaneously determining five predominant odors, namely, dimethyltrisulfide, 2-methylisoborneol, geosmin, β-cyclocitral and β-ionone, from the primary sources and sinks is demonstrated. This instrument facilitates the extraction and concentration of odors from quite different matrices simultaneously. This device is a solvent-free automated system that does not require cleaning and is timesaving. The calibration curves of the five odor compounds showed good linearity in the range of 1-500 ng/L, with correlation coefficients above 0.999 (levels=7) and with residuals ranging from approximately 77% to 104%. The limits of detection (S/N=3) were below 0.15 ng/L in algae sample and 0.07 ng/g in sediment and fish tissue samples. The relative standard deviations were between 2.65% and 7.29% (n=6). Thus the proposed design is ready for rapid translation into a standard analytical tool and is useful for multiple applications in the analysis of off-flavors.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
β-Ionone, predominantly trans, ≥97%, FCC, FG
Sigma-Aldrich
β-Ionone, 96%
Sigma-Aldrich
β-Ionone, natural, ≥95%, FG
Sigma-Aldrich
2-Methylisoborneol, ≥98.0% (GC)
Sigma-Aldrich
2-Methylisoborneol solution, ~10 mg/mL in methanol, ≥98% (GC)