• Home
  • Search Results
  • A new biological test utilising the yeast Saccharomyces cerevisiae for the rapid detection of toxic substances in water.

A new biological test utilising the yeast Saccharomyces cerevisiae for the rapid detection of toxic substances in water.

Environmental toxicology and pharmacology (2012-03-13)
Lubomira Rumlova, Jaroslava Dolezalova
ABSTRACT

This study evaluates the toxic effects of five substances (atropine, fenitrothion, potassium cyanide, mercuric chloride and lead nitrate) on the yeast Saccharomyces cerevisiae. It describes a new biological toxicity test based on inhibition of S. cerevisiae viability and compares it with two standard toxicity tests based on Daphnia magna mobility inhibition (EN ISO 6341) and Vibrio fischeri bioluminiscence inhibition (EN ISO 11348-2). The new biological test -S. cerevisiae lethal test - is cheaper and 24 times faster than the D. magna test. The test speed is comparable with the V. fischeri test but the new test is more sensitive for some substances. The test indicates reliably the presence of all used toxicants in water in concentrations which are significantly lower than the concentration in toxic or lethal doses for man. Therefore, this new toxicity test could be proposed for rapid detection of toxic substances in water.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lead(II) nitrate, ACS reagent, ≥99.0%
Sigma-Aldrich
Potassium cyanide, BioUltra, ≥98.0% (AT)
Sigma-Aldrich
Potassium cyanide, ACS reagent, ≥96.0%
Sigma-Aldrich
Potassium cyanide, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥97.0%
Sigma-Aldrich
Lead(II) nitrate, 99.999% trace metals basis
Supelco
Fenitrothion, PESTANAL®, analytical standard
Sigma-Aldrich
Lead(II) nitrate, ≥99.95% trace metals basis
Sigma-Aldrich
Potassium cyanide, technical, ≥96%
Sigma-Aldrich
Potassium cyanide-15N, 98 atom % 15N