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Toxicity of metalaxyl, azoxystrobin, dimethomorph, cymoxanil, zoxamide and mancozeb to Phytophthora infestans isolates from Serbia.

Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes (2012-03-20)
Emil Rekanović, Ivana Potočnik, Svetlana Milijašević-Marčić, Miloš Stepanović, Biljana Todorović, Milica Mihajlović
ABSTRACT

A study of the in vitro sensitivity of 12 isolates of Phytophthora infestans to metalaxyl, azoxystrobin, dimethomorph, cymoxanil, zoxamide and mancozeb, was conducted. The isolates derived from infected potato leaves collected at eight different localities in Serbia during 2005-2007. The widest range of EC(50) values for mycelial growth of the isolates was recorded for metalaxyl. They varied from 0.3 to 3.9 μg mL(-1) and were higher than those expected in a susceptible population of P. infestans. The EC(50) values of the isolates were 0.16-0.30 μg mL(-1) for dimethomorph, 0.27-0.57 μg mL(-1) for cymoxanil, 0.0026-0.0049 μg mL(-1) for zoxamide and 2.9-5.0 μg mL(-1) for mancozeb. The results indicated that according to effective concentration (EC(50)) the 12 isolates of P. infestans were sensitive to azoxystrobin (0.019-0.074 μg mL(-1)), and intermediate resistant to metalaxyl, dimethomorph and cymoxanil. According to resistance factor, all P. infestans isolates were sensitive to dimethomorph, cymoxanil, mancozeb and zoxamide, 58.3% of isolates were sensitive to azoxystrobin and 50% to metalaxyl. Gout's scale indicated that 41.7% isolates were moderately sensitive to azoxystrobin and 50% to metalaxyl.

MATERIALS
Product Number
Brand
Product Description

Supelco
Mancozeb, PESTANAL®, analytical standard
Supelco
Metalaxyl, PESTANAL®, analytical standard
Supelco
Cymoxanil, PESTANAL®, analytical standard
Supelco
Dimethomorph, PESTANAL®, analytical standard
Supelco
Maneb, PESTANAL®, analytical standard
Supelco
Zineb, PESTANAL®, analytical standard
Metalaxyl, analytical standard