• Home
  • Search Results
  • Fast and sensitive dye-sensor based on fluorescein/reduced graphene oxide complex.

Fast and sensitive dye-sensor based on fluorescein/reduced graphene oxide complex.

The Analyst (2012-05-01)
Sheng Tian Huang, Yan Shi, Nian Bing Li, Hong Qun Luo

We report on a fast, sensitive, label-free, and general dye-sensor platform for synthetic organic dyes detection by competitive adsorption on reduced graphene oxide (rGO) against a fluorescent dye (FD). Fluorescein (Fl) as fluorescence indicator and a cationic dye methylene blue (MB) as model analyte were employed to investigate the analytical feature of this assay platform. An anionic dye sunset yellow FCF (SY) was chosen as a comparison analyte to test the generality of this strategy. Results show that rGO can bind Fl and quench the fluorescence by fluorescence resonance energy transfer (FRET), while MB can displace Fl quickly from the Fl/rGO complex by competitive adsorption, inducing the fluorescence recovery which provides a quantitative readout for MB. Besides, this design was simply based on the competitive adsorption of rGO between dye and FD, and can be generally applied to other dyes for label-free detection. The fluorescence enhancement efficiency (FEE) is proportional to the dye concentration over the range of 7.60-420.00 ng mL(-1) MB and 7.28-400.25 ng mL(-1) SY, respectively. The linear regression equations were calculated as FEE(MB) = 0.0192c(MB)- 0.3103 for MB and FEE(SY) = 0.0142 c(SY)- 0.0427 for SY, with the detection limits of 1.03 and 1.15 ng mL(-1), respectively. The MB in waste water and SY in an orange-flavored sports drink sample were assayed with satisfactory results.

Product Number
Product Description

Sunset Yellow FCF, Dye content 90 %
Sunset Yellow FCF, analytical standard

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.