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Methanol-driven enhanced biological phosphorus removal with a syntrophic consortium.

Biotechnology and bioengineering (2012-08-14)
Carlota Tayà, Javier Guerrero, Gianni Vanneste, Albert Guisasola, Juan A Baeza

The presence of suitable carbon sources for enhanced biological phosphorus removal (EBPR) plays a key role in phosphorus removal from wastewater in urban WWTP. For wastewaters with low volatile fatty acids (VFAs) content, an external carbon addition is necessary. As methanol is the most commonly external carbon source used for denitrification it could be a priori a promising alternative, but previous attempts to use it for EBPR have failed. This study is the first successful report of methanol utilization as external carbon source for EBPR. Since a direct replacement strategy (i.e., supply of methanol as a sole carbon source to a propionic-fed PAO-enriched sludge) failed, a novel process was designed and implemented successfully: development of a consortium with anaerobic biomass and polyphosphate accumulating organisms (PAOs). Methanol-degrading acetogens were (i) selected against other anaerobic methanol degraders from an anaerobic sludge; (ii) subjected to conventional EBPR conditions (anaerobic + aerobic); and (iii) bioaugmented with PAOs. EBPR with methanol as a sole carbon source was sustained in a mid-term basis with this procedure.

Product Number
Product Description

Propionic acid, ACS reagent, ≥99.5%
Propionic acid, ≥99.5%
Propionic acid, analytical standard
Propionic acid, ≥99.5%, FCC, FG
Propionic acid, natural, 99%, FG
Propionic acid, puriss. p.a., ≥99.5% (GC)
Propionic acid, BioReagent, suitable for insect cell culture, ~99%