MilliporeSigma

4Pi Microscopy.

Methods in molecular biology (Clifton, N.J.) (2012-10-23)
Roman Schmidt, Johann Engelhardt, Marion Lang
ABSTRACT

Optical microscopy has become a key technology in the life sciences today. Its noninvasive nature provides access to the interior of intact and even living cells, where specific molecules can be precisely localized by fluorescent tagging. However, the attainable 3D resolution of an optical microscope has long been hampered by a comparatively poor resolution along the optic axis. By coherent focusing through two objective lenses, 4Pi microscopy improves the axial resolution by three- to fivefold. This primer is intended as a starting point for the design and operation of a 4Pi microscope of type A.

MATERIALS
Product Number
Brand
Product Description

Supelco
Thiodiglycol solution, 1000 μg/mL in methanol, ampule of 1.2 mL, certified reference material, Cerilliant®
Sigma-Aldrich
2,2′-Thiodiethanol, ≥99%
Sigma-Aldrich
2,2′-Thiodiethanol, purum, ≥95.0% (GC)