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Evaluation of deoxyribonucleic acid toxicity induced by the radiopharmaceutical 99mTechnetium-Methylenediphosphonic acid and by stannous chloride in Wistar rats.

Molecules (Basel, Switzerland) (2012-11-03)
José Carlos Pelielo De Mattos, Vanessa Coutinho de Matos, Michelle Pinheiro Rodrigues, Marcia Betânia Nunes de Oliveira, Flavio José S Dantas, Sebastião David Santos-Filho, Mario Bernardo-Filho, Adriano Caldeira-de-Araujo
ABSTRACT

Radiopharmaceuticals are employed in patient diagnostics and disease treatments. Concerning the diagnosis aspect, technetium-99m (99mTc) is utilized to label radiopharmaceuticals for single photon computed emission tomography (SPECT) due to its physical and chemical characteristics. 99mTc fixation on pharmaceuticals depends on a reducing agent, stannous chloride (SnCl(2)) being the most widely-utilized. The genotoxic, clastogenic and anegenic properties of the 99mTc-MDP(methylene diphosphonate used for bone SPECT) and SnCl(2) were evaluated in Wistar rat blood cells using the Comet assay and micronucleus test. The experimental approach was to endovenously administer NaCl 0.9% (negative control), cyclophosphamide 50 mg/kg b.w. (positive control), SnCl(2) 500 μg/mL or 99mTc-MDP to animals and blood samples taken immediately before the injection, 3, and 24 h after (in the Comet assay) and 36 h after, for micronucleus test. The data showed that both SnCl(2) and 99mTc-MDP-induced deoxyribonucleic acid (DNA) strand breaks in rat total blood cells, suggesting genotoxic potential. The 99mTc-MDP was not able to induce a significant DNA strand breaks increase in in vivo assays. Taken together, the data presented here points to the formation of a complex between SnCl(2) in the radiopharmaceutical 99mTc-MDP, responsible for the decrease in cell damage, compared to both isolated chemical agents. These findings are important for the practice of nuclear medicine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Tin(II) chloride, reagent grade, 98%
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Tin(II) chloride dihydrate, ACS reagent, 98%
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Tin(II) chloride dihydrate, ≥99.995% trace metals basis
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Tin(II) chloride dihydrate, reagent grade, 98%
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Tin(II) chloride, anhydrous, powder, ≥99.99% trace metals basis
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Tin(II) chloride, ≥99.99% trace metals basis
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Tin(II) chloride dihydrate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥98%
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Tin(II) chloride dihydrate, ≥99.99% trace metals basis
Supelco
Tin(II) chloride dihydrate, suitable for AAS