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Gene expression patterns and life cycle responses of toxicant-exposed chironomids.

Environmental science & technology (2012-11-07)
Marino Marinković, Wim C de Leeuw, Wim A Ensink, Mark de Jong, Timo M Breit, Wim Admiraal, Michiel H S Kraak, Martijs J Jonker

Cellular stress responses are frequently presumed to be more sensitive than traditional ecotoxicological life cycle end points such as survival and growth. Yet, the focus to reduce test duration and to generate more sensitive end points has caused transcriptomics studies to be performed at low doses during short exposures, separately and independently from traditional ecotoxicity tests, making comparisons with life cycle end points indirect. Therefore we aimed to directly compare the effects on growth, survival, and gene expression of the nonbiting midge Chironomus riparius. To this purpose, we simultaneously analyzed life cycle and transcriptomics responses of chironomid larvae exposed to four model toxicants. We observed that already at the lowest test concentrations many transcripts were significantly differentially expressed, while the life cycle end points of C. riparius were hardly affected. Analysis of the differentially expressed transcripts showed that at the lowest test concentrations substantial and biologically relevant cellular stress was induced and that many transcripts responded already maximally at these lowest test concentrations. The direct comparison between molecular end life cycle responses after fourteen days of exposure revealed that gene expression is more sensitive to toxicant exposure than life cycle end points, underlining the potential of transcriptomics for ecotoxicity testing and environmental risk assessment.

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Phenanthrene, 98%
Phenanthrene, analytical standard, for environmental analysis
Phenanthrene, sublimed grade, ≥99.5%
Phenanthrene, certified reference material, TraceCERT®
Phenanthrene solution, certified reference material, 5000 μg/mL in methanol