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Improved herbivore resistance in cultivated tomato with the sesquiterpene biosynthetic pathway from a wild relative.

Proceedings of the National Academy of Sciences of the United States of America (2012-11-22)
Petra M Bleeker, Rossana Mirabella, Paul J Diergaarde, Arjen VanDoorn, Alain Tissier, Merijn R Kant, Marcel Prins, Martin de Vos, Michel A Haring, Robert C Schuurink
ABSTRACT

Tomato breeding has been tremendously efficient in increasing fruit quality and quantity but did not focus on improving herbivore resistance. The biosynthetic pathway for the production of 7-epizingiberene in a wild tomato was introduced into a cultivated greenhouse variety with the aim to obtain herbivore resistance. 7-Epizingiberene is a specific sesquiterpene with toxic and repellent properties that is produced and stored in glandular trichomes. We identified 7-epizingiberene synthase (ShZIS) that belongs to a new class of sesquiterpene synthases, exclusively using Z-Z-farnesyl-diphosphate (zFPP) in plastids, probably arisen through neo-functionalization of a common ancestor. Expression of the ShZIS and zFPP synthases in the glandular trichomes of cultivated tomato resulted in the production of 7-epizingiberene. These tomatoes gained resistance to several herbivores that are pests of tomato. Hence, introduction of this sesquiterpene biosynthetic pathway into cultivated tomatoes resulted in improved herbivore resistance.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Farnesyl pyrophosphate ammonium salt, methanol:ammonia solution, ≥95% (TLC)

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