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Microfluidic channel with embedded SERS 2D platform for the aptamer detection of ochratoxin A.

Analytical and bioanalytical chemistry (2012-11-29)
Betty C Galarreta, Mohammadali Tabatabaei, Valérie Guieu, Eric Peyrin, François Lagugné-Labarthet
ABSTRACT

A selective aptameric sequence is adsorbed on a two-dimensional nanostructured metallic platform optimized for surface-enhanced Raman spectroscopy (SERS) measurements. Using nanofabrication methods, a metallic nanostructure was prepared by electron-beam lithography onto a glass coverslip surface and embedded within a microfluidic channel made of polydimethylsiloxane, allowing one to monitor in situ SERS fingerprint spectra from the adsorbed molecules on the metallic nanostructures. The gold structure was designed so that its localized surface plasmon resonance matches the excitation wavelength used for the Raman measurement. This optofluidic device is then used to detect the presence of a toxin, namely ochratoxin-A (OTA), in a confined environment, using very small amounts of chemicals, and short data acquisition times, by taking advantage of the optical properties of a SERS platform to magnify the Raman signals of the aptameric monolayer system and avoiding chemical labeling of the aptamer or the OTA target.

MATERIALS
Product Number
Brand
Product Description

Supelco
Ochratoxin A solution, 10 μg/mL in acetonitrile, analytical standard
Sigma-Aldrich
Ochratoxin A, from Petromyces albertensis, ≥98% (HPLC)
Supelco
Ochratoxin A, from Aspergillus ochraceus, reference material