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[Determination of sixteen food additives in beverage by capillary zone electrophoresis].

Se pu = Chinese journal of chromatography (2012-11-30)
Weiran Long, Yihong Cen, Xingyi Wang, Yu Bai, Huwei Liu

A capillary zone electrophoretic (CZE) method for the separation of ten synthetic colorants such as acid red 92, patent blue V, uranine, acid red 1, indigo carmine, black BN, ponceau 6R, quinoline, amaranth, lemon yellow, and six preservatives including benzoic acid, sorbic acid, methyl paraben, ethyl paraben, propyl paraben, butyl paraben in beverages was developed. The effects of separation voltage, capillary temperature, pH and concentration of running buffer on the separation were investigated. A 46 cm x 50 microm uncoated fused silica capillary was used with 70 mmol/L boric acid (pH 9.5) containing 4% (v/v) acetonitrile as background electrolyte solution. The detection wavelength was set at 220 nm, and the run voltage was 30 kV. The injection time was 5 s at 500 mPa and the temperature was 25 degrees C. The linear relationship between the mass concentration and peak area for each of these analytes was obtained in

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Product Description

Patent Blue VF, Dye content 50 %
Indigo carmine, analytical standard
Indigo carmine, certified by the Biological Stain Commission, Dye content 85 %
Patent blue VF sodium salt, for microscopy
Indigo carmine, for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)