• Home
  • Search Results
  • Evaluation of abiotic stress tolerance in transgenic potato plants with reduced expression of PSII manganese stabilizing protein.

Evaluation of abiotic stress tolerance in transgenic potato plants with reduced expression of PSII manganese stabilizing protein.

Plant science : an international journal of experimental plant biology (2012-12-04)
Mayank Anand Gururani, Chandrama Prakash Upadhyaya, Reto J Strasser, Jae Woong Yu, Se Won Park
ABSTRACT

Manganese stabilizing protein (MSP) is an important component of the Photosystem II (PSII) oxygen evolving complex. In our previous work, transgenic potato plants with reduced expression of MSP (MSP-As) were developed and their physiological and biochemical responses were studied. In this report, we address the response of MSP-As plants toward salinity, heavy metal and osmotic stresses. MSP-As plants treated with NaCl, ZnCl(2) or mannitol solution showed significant level of tolerance under all the stress conditions. Specific enzyme activities of major ROS-scavenging enzymes were found significantly higher in MSP-As plants than the control plants. MSP-As plants accumulated increased levels of proline and low molecular weight metabolites such as ascorbate and α-tocopherol, which indicated that these plants were much more resistant to stress compared to the corresponding control plants. The primary photochemical efficiencies and the OJIP kinetics analyses further confirmed that MSP-As plants were in better optimal health under stress compared to the control plants. Although the exact reason behind the increased stress tolerance in stressed MSP-As plants is unclear, our results strongly indicate the role of MSP of unknown function in abiotic stress tolerance.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Zinc chloride, reagent grade, ≥98%
Sigma-Aldrich
Zinc chloride, 99.999% trace metals basis
Sigma-Aldrich
Zinc chloride, anhydrous, powder, ≥99.995% trace metals basis
Sigma-Aldrich
Zinc chloride, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Zinc chloride 0.1 M solution
Sigma-Aldrich
Zinc chloride solution, 1.9 M in 2-methyltetrahydrofuran
Sigma-Aldrich
Zinc chloride solution, 0.5 M in THF
Sigma-Aldrich
Zinc chloride solution, 1.0 M in diethyl ether
Sigma-Aldrich
Zinc chloride, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Zinc chloride, ACS reagent, ≥97%
Sigma-Aldrich
Zinc chloride, puriss., meets analytical specification of Ph. Eur., BP, USP, 98-100.5%
Sigma-Aldrich
Zinc chloride, AnhydroBeads, amorphous, −10 mesh, 99.99% trace metals basis
Sigma-Aldrich
Zinc chloride, AnhydroBeads, amorphous, −10 mesh, 99.999% trace metals basis

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.