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[The influence of different processing on the content of flavones of Dendranthema morifolium].

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (2012-12-12)
Chuan-shan Jin, De-lin Wu, Nian-jun Yu, Hui-fang Chen, Tong-sheng Wang, Wei Zhang
ABSTRACT

To investigate the effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin, and acacetin of Dendranthema morifolium. The optimal conditions were achieved on phenomenex, synergi Fusion-RP (5 microm, 4.6 mm x 250 mm)analytical column with a gradient mobile phase consisting of methanol and 0.1% phosphoric acid and detection wavelength set at 350 nm. The significant effect of different processing methods on the content of luteolin-7-O-beta-D-glucoside, acacetin-7-O-beta-D-glucoside, luteolin and acacetin was proved. Different processing methods have significant effect on flavonoids of Dendranthema morifolium, it should be paid attention to drying temperature while sulphur fumigation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Luteolin, ≥98% (TLC), powder
Supelco
Luteolin, analytical standard
Supelco
Luteolin 7-glucoside, analytical standard
Sigma-Aldrich
Luteolin 7-O-β-D-glucoside, ≥98.0% (HPLC)
Sigma-Aldrich
Acacetin, ≥97.0% (HPLC)
Luteolin, primary reference standard
Luteolin 7-glucoside, primary reference standard