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Optimization of selenylation conditions for Chinese angelica polysaccharide based on immune-enhancing activity.

Carbohydrate polymers (2012-12-12)
Tao Qin, Jin Chen, Deyun Wang, Yuanliang Hu, Mi Wang, Jing Zhang, The Luong Nguyen, Cui Liu, Xu Liu
ABSTRACT

Chinese angelica polysaccharide (CAP) was extracted by water decoction and ethanol precipitation, purified through eliminating protein by Sevage method and column chromatography of Sephadex G-200, then selenizingly modified by nitric acid-sodium selenite method according to L(9)(3(4)) orthogonal design of three-factors, the usage amount of sodium selenite, reaction temperature and reaction time, at three level to obtain nine selenizing CAPs, sCAP(1)-sCAP(9). Their effects on chicken peripheral lymphocytes proliferation in vitro were compared by MTT assay taking the non-modified CAP as control. The results showed that selenylation modification could significantly enhance the immune-enhancing activity of CAP, sCAP(2) presented best effect and the optimal modification conditions were 200mg of sodium selenite for 500 mg of CAP, the reaction temperature of 70 °C and the reaction time of 6 h.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nitric acid, ACS reagent, 70%
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Nitric acid, red, fuming, HNO3 >90 %
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Nitric acid, puriss. p.a., ≥65% (T)
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Sodium selenite, anhydrous, ≥90.0% (RT)
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Sodium hydrogen selenite, purum p.a., ≥96.0% (RT)