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Toxoflavin lyase enzyme as a marker for selecting potato plant transformants.

Bioscience, biotechnology, and biochemistry (2012-12-12)
Mi-Sun Kim, HyunSoon Kim, Jae Sun Moon, Ingyu Hwang, Hyouk Joung, Jae-Heung Jeon
ABSTRACT

This study established a new system for potato transformation using toxoflavin as selection agent and toxoflavin lyase (tflA) as selectable marker gene. Potato plants expressing tflA was successfully transformed on toxoflavin medium with 27% efficiency, similar to that for the hygromycin/hpt selection system. The transgenic potato expressing tflA also showed resistance to Burkholderia glumea infection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
PKF118-310, ≥98% (HPLC)
Sigma-Aldrich
[D-Trp7, Ala8, D-Phe10]-α-Melanocyte Stimulating Hormone Amide Fragment 6-11, ≥97% (HPLC)