• Home
  • Search Results
  • Hyper alginate gel microbead formation by molecular diffusion at the hydrogel/droplet interface.

Hyper alginate gel microbead formation by molecular diffusion at the hydrogel/droplet interface.

Langmuir : the ACS journal of surfaces and colloids (2012-12-14)
Hirotada Hirama, Taisuke Kambe, Kyouhei Aketagawa, Taku Ota, Hiroyuki Moriguchi, Toru Torii
ABSTRACT

We report a simple method for forming monodispersed, uniformly shaped gel microbeads with precisely controlled sizes. The basis of our method is the placement of monodispersed sodium alginate droplets, formed by a microfluidic device, on an agarose slab gel containing a high-osmotic-pressure gelation agent (CaCl(2) aq.): (1) the droplets are cross-linked (gelated) due to the diffusion of the gelation agent from the agarose slab gel to the sodium alginate droplets and (2) the droplets simultaneously shrink to a fraction of their original size (<100 μm in diameter) due to the diffusion of water molecules from the sodium alginate droplets to the agarose slab gel. We verified the mass transfer mechanism between the droplet and the agarose slab gel. This method circumvents the limitations of gel microbead formation, such as the need to prepare microchannels of various sizes, microchannel clogging, and the deformation of the produced gel microbeads.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Calcium chloride dihydrate, ACS reagent, ≥99%
Sigma-Aldrich
Calcium chloride solution, BioUltra, for molecular biology, ~1 M in H2O
Sigma-Aldrich
Calcium chloride dihydrate, for molecular biology, ≥99.0%
Sigma-Aldrich
Calcium chloride dihydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
Sigma-Aldrich
Calcium chloride, meets USP testing specifications
Sigma-Aldrich
Calcium chloride, anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%
Sigma-Aldrich
Calcium chloride, anhydrous, powder, 99.99% trace metals basis
Sigma-Aldrich
Calcium chloride dihydrate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Calcium chloride dihydrate, BioXtra, ≥99.0%
Sigma-Aldrich
Calcium chloride dihydrate, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%
Sigma-Aldrich
Calcium chloride, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
Supelco
Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
Sigma-Aldrich
Calcium chloride dihydrate, BioUltra, for molecular biology, ≥99.5% (KT)
Sigma-Aldrich
Calcium chloride dihydrate, puriss., meets analytical specification of Ph. Eur., USP, FCC, E509, 99-103%, ≤0.0001% Al
Supelco
Calcium ion solution for ISE, 0.1 M Ca, analytical standard (for ion-selective electrodes)
Sigma-Aldrich
Calcium chloride dihydrate, tested according to Ph. Eur.
Sigma-Aldrich
Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis