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Longitudinal study of [D10]phenanthrene metabolism by the diol epoxide pathway in smokers.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals (2013-01-23)
Stephen S Hecht, J Bradley Hochalter, Steven G Carmella, Yan Zhang, Diane M Rauch, Naomi Fujioka, Joni Jensen, Dorothy K Hatsukami
ABSTRACT

The extent of metabolism of [D10]phenanthrene to [D(10)]r-1,t-2,3,c-4-tetrahydroxy-1,2,3,4-tetradeuterophenanthrene ([D10]PheT) could be a biomarker of human metabolic activation of carcinogenic polycyclic aromatic hydrocarbons, leading to identification of smokers particularly susceptible to lung cancer. The longitudinal stability of [D10]PheT was evaluated in 24 cigaret smokers given 7-8 oral doses of [D10]phenanthrene (10 µg) over 5.5 months. [D10]PheT in 6 h urine was quantified after each dose. The overall coefficient of variation for 24 subjects was (mean ± S.D.) 27.4% ± 8.83%. Thus, a single administration of [D10]phenanthrene is likely sufficient to determine a smoker's ability to metabolize it to [D10]PheT.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phenanthrene, 98%
Supelco
Phenanthrene, analytical standard, for environmental analysis
Sigma-Aldrich
Phenanthrene, sublimed grade, ≥99.5%
Supelco
Phenanthrene, certified reference material, TraceCERT®
Supelco
Phenanthrene solution, certified reference material, 5000 μg/mL in methanol