MilliporeSigma

Effect of silk protein processing on drug delivery from silk films.

Macromolecular bioscience (2013-01-26)
Eleanor M Pritchard, Xiao Hu, Violet Finley, Catherine K Kuo, David L Kaplan
ABSTRACT

Sericin removal from the core fibroin protein of silkworm silk is a critical first step in the use of silk for biomaterial-related applications, but degumming can affect silk biomaterial properties, including molecular weight, viscosity, diffusivity and degradation behavior. Increasing the degumming time (10, 30, 60, and 90 min) decreases the average molecular weight of silk protein in solution, silk solution viscosity, and silk film glass-transition temperature, and increases the rate of degradation of a silk film by protease. Model compounds spanning a range of physical-chemical properties generally show an inverse relationship between degumming time and release rate through a varied degumming time silk coating. Degumming provides a useful control point to manipulate silk's material properties.

MATERIALS
Product Number
Brand
Product Description

Supelco
Indigo carmine, analytical standard
Sigma-Aldrich
Indigo carmine, certified by the Biological Stain Commission, Dye content 85 %
Sigma-Aldrich
Reactive Red 120
Sigma-Aldrich
Indigo carmine, for microscopy (Bact., Hist.), indicator (pH 11.5-14.0)