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Simultaneous determination of aflatoxin B(1) and ochratoxin A in licorice roots and fritillary bulbs by solid-phase extraction coupled with high-performance liquid chromatography-tandem mass spectrometry.

Food chemistry (2013-02-16)
Lizhi Wang, Zhen Wang, Weiwei Gao, Juan Chen, Meihua Yang, Ying Kuang, Linfang Huang, Shilin Chen
ABSTRACT

An effective method was developed for screening licorice roots and fritillary bulbs for contamination by aflatoxin B(1) and ochratoxin A using high-performance liquid chromatography connected to tandem mass spectrometry (HPLC-MS/MS). The samples were pre-concentrated and purified using solid-phase extraction, which provided satisfactory results. The separation was performed on a Waters Xbridge(TM) C18 column with a linear gradient of acetonitrile - water containing 5mM ammonium acetate. The MS spectrum was acquired in positive mode with both single quadrupole (Q1) and multiple reaction monitoring (MRM). The optimised method offered a good linear correlation (r(2)>0.9967), excellent precision (RSD<2.83%) and acceptable recovery (from 92.78 to 105.37%). The limits of detection (LOD) and the limits of quantification (LOQ) were less than 0.024 μg/kg and 0.095 μg/kg, respectively. The validated method was successfully applied to the rapid screening for AFB(1) and OTA in licorice roots and fritillary bulbs.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Aflatoxin B1 from Aspergillus flavus, from Aspergillus flavus
Supelco
Ochratoxin A solution, 10 μg/mL in acetonitrile, analytical standard
Sigma-Aldrich
Ochratoxin A, from Petromyces albertensis, ≥98% (HPLC)
Supelco
Aflatoxin B1 solution, 2 μg/mL in acetonitrile, analytical standard
Supelco
Ochratoxin A, from Aspergillus ochraceus, reference material
Aflatoxin B1 solution, 3.79 μg/g in acetonitrile, ERM®, certified reference material