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  • Hierarchical polymer brush nanoarrays: a versatile way to prepare multiscale patterns of proteins.

Hierarchical polymer brush nanoarrays: a versatile way to prepare multiscale patterns of proteins.

ACS applied materials & interfaces (2013-02-23)
Yunfeng Li, Junhu Zhang, Wendong Liu, Daowei Li, Liping Fang, Hongchen Sun, Bai Yang
ABSTRACT

This paper presents a versatile way to prepare multiscale and gradient patterns of proteins. The protein patterns are fabricated by conjugating proteins covalently on patterns of polymer brush that are prepared by techniques combining colloidal lithography with photolithography, and two-step colloidal lithography. Taking advantages of this technique, the parameters of protein patterns, such as height, diameters, periods, and distances between two dots, can be arbitrarily tuned. In addition, the protein patterns with varies of architectures, such as microdiscs, microstripes, microrings, microtriangles, microgrids, etc., consisting of protein nanodots, are prepared and the sample size is up to 4 cm(2). The as-prepared patterns of fibronectin can promote the cell adhesion and cell location.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), BioReagent, powder, suitable for cell culture
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 20,000
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 1,000,000
Sigma-Aldrich
Poly(2-hydroxyethyl methacrylate), average Mv 300,000, crystalline