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Monitoring chronic physical stress using biomarkers, performance protocols and mathematical functions to identify physiological adaptations in rats.

Laboratory animals (2013-03-08)
Gustavo Gomes de Araujo, Marcelo Papoti, Fúlvia de Barros Manchado-Gobatto, Maria Alice Rostom de Mello, Claudio Alexandre Gobatto
ABSTRACT

This study was undertaken to characterize the effects of monotonous training at lactate minimum (LM) intensity on aerobic and anaerobic performances; glycogen concentrations in the soleus muscle, the gastrocnemius muscle and the liver; and creatine kinase (CK), free fatty acids and glucose concentrations in rats. The rats were separated into trained (n = 10), baseline (n = 10) and sedentary (n = 10) groups. The trained group was submitted to the following: 60 min/day, 6 day/week and intensity equivalent to LM during the 12-week training period. The training volume was reduced after four weeks according to a sigmoid function. The total CK (U/L) increased in the trained group after 12 weeks (742.0 ± 158.5) in comparison with the baseline (319.6 ± 40.2) and the sedentary (261.6 ± 42.2) groups. Free fatty acids and glycogen stores (liver, soleus muscle and gastrocnemius muscle) increased after 12 weeks of monotonous training but aerobic and anaerobic performances were unchanged in relation to the sedentary group. The monotonous training at LM increased the level of energy substrates, unchanged aerobic performance, reduced anaerobic capacity and increased the serum CK concentration; however, the rats did not achieve the predicted training volume.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glycogen from bovine liver, ≥85%
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Glycogen from Mytilus edulis (Blue mussel), for DNA precipitations
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Glycogen from oyster, ≥75% dry basis
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Glycogen from rabbit liver, ≥85% dry basis (enzymatic)
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Glycogen from Mytilus edulis (Blue mussel), ≥85%
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Glycogen from oyster, Type XI