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  • Impact of arachidonic acid enrichment of live rotifer prey on bacterial communities in rotifer and larval fish cultures.

Impact of arachidonic acid enrichment of live rotifer prey on bacterial communities in rotifer and larval fish cultures.

Canadian journal of microbiology (2013-04-02)
Laurent H Seychelles, Kim Doiron, Céline Audet, Réjean Tremblay, Fabrice Pernet, Karine Lemarchand
ABSTRACT

Rotifers (Brachionus plicatilis), commonly used at first feeding in commercial fish hatcheries, carry a large bacteria load. Because they are relatively poor in essential fatty acids, it is common practice to enrich them with fatty acids, including arachidonic acid (AA). This study aims to determine whether prey enrichment with AA may act as a prebiotic and modify the microbial community composition either in AA-enriched rotifer cultures or in larval-rearing water using winter flounder (Pseudopleuronectes americanus) as a larval fish model. AA enrichment modified the bacterial community composition in both the rotifer culture tanks and the larval-rearing tanks. We observed an increase in the number of cultivable bacteria on TCBS (thiosulfate-citrate-bile salts-sucrose) agar, used as a proxy for the abundance of Vibrio sp. The results suggest that AA may also play an indirect role in larval health.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Arachidonic acid, from non-animal source, ≥98.5% (GC)
Millipore
Gram Staining Kit, for microscopy, used for differentiation of bacteria on the basis of their gram nature.
Sigma-Aldrich
Arachidonic acid, >95.0% (GC)