Photolytic covalent binding of indoleacrylic acid to DNA.

Photochemistry and photobiology (1990-03-01)
S J Farrow, T Mohammad, W Baird, H Morrison
ABSTRACT

E-beta-Indol-3-ylacrylic acid (IA), radiolabelled at the 2 position with 14C, and calf thymus DNA have been irradiated with UV light (lambda greater than 280 nm) in phosphate buffer (pH 7.0). Re-isolation of the DNA indicates covalent binding of IA at levels of up to 295 nmol IA/mg native DNA (0.097 IA/base). Binding is observed for both native and heat denatured DNA, but is more efficient with the latter. Quantum efficiencies of 2.60 X 10(-5) and 2.30 X 10(-4) mol IA bound to native DNA/mol photon absorbed have been measured at 308 and 266 nm, respectively. Studies with the four polyribonucleotides indicate a strong preference for binding to poly[U]. Photolysis of either untreated or enzymatically degraded labeled native DNA with 254 nm light leads to the reformation of IA, and a 2 + 2 photocycloadduct of IA and thymidine has been isolated and characterized and matched by HPLC to a DNA derived adduct. Equilibrium dialysis studies provide no evidence for preassociation of IA to DNA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
3-Indoleacrylic acid, BioReagent, ≥98% (HPLC)
Sigma-Aldrich
trans-3-Indoleacrylic acid, 98%
Sigma-Aldrich
3-Indoleacrylic acid, powder

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