MilliporeSigma
  • Home
  • Search Results
  • Evidence of small modulation of ethinylestradiol induced effects by concurrent exposure to trenbolone in male eelpout Zoarces viviparus.

Evidence of small modulation of ethinylestradiol induced effects by concurrent exposure to trenbolone in male eelpout Zoarces viviparus.

Environmental pollution (Barking, Essex : 1987) (2013-04-16)
Yohana M Velasco-Santamaría, Poul Bjerregaard, Bodil Korsgaard
ABSTRACT

The interaction of xenobiotics is common in aquatic ecosystems; therefore, we wanted to evaluate if trenbolone (TB) modulates the effects of 17α-ethinylestradiol (EE2). Male eelpout (Zoarces viviparus) were exposed to 5 ng L(-1) EE2 continuously for 19 d (EE2-C) or discontinuously (11 d, EE2-D) alone or in combination with low (50 ng L(-1), TBL) or high (500 ng L(-1), TBH) concentrations of TB (19 d). Exposure to EE2 caused reduced gonadosomatic index, increased plasma vitellogenin concentrations, up-regulated vtg and era mRNA expression and severe alterations in gonadal histology. TBL and TBH did not affect plasma vitellogenin, era or vtg mRNA expression. TBL and TBH did not counteract the EE2-induced increase in plasma vitellogenin and reduction in 11-ketotestosterone whereas TBH counteracted the EE2 induced increase in vtg and era mRNA expression. Exposure to TBH and EE2-C + TBH lead to severe gonadal histology alterations. TBL and EE2-D + TBH exposed fish showed less histopathological alterations.

MATERIALS
Product Number
Brand
Product Description

Supelco
Trenbolone, analytical standard, for drug analysis
Sigma-Aldrich
11-Ketotestosterone
Sigma-Aldrich
17α-Ethynylestradiol, ≥98%
Supelco
Trenbolone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
Ethinylestradiol, European Pharmacopoeia (EP) Reference Standard
Supelco
17α-Ethynylestradiol solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Ethinylestradiol for system suitability, European Pharmacopoeia (EP) Reference Standard