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Mesoporous silicas synthesis and application for lignin peroxidase immobilization by covalent binding method.

Journal of environmental sciences (China) (2013-04-17)
Zunfang Hu, Longqian Xu, Xianghua Wen
ABSTRACT

Immobilization of enzymes on mesoporous silicas (MS) allows for good reusability. MS with two-dimensional hexagonal pores in diameter up to 14.13 nm were synthesized using Pluronic P123 as template and 1,3,5-triisopropylbenzene as a swelling agent in acetate buffer. The surface of MS was modified by the silanization reagents 3-aminopropyltriethoxysilane. Lignin peroxidase (LiP) was successfully immobilized on the modified MS through covalent binding method by four agents: glutaraldehyde, 1,4-phenylene diisothiocyanate, cyanotic chloride and water-soluble carbodiimide. Results showed that cyanotic chloride provided the best performance for LIP immobilization. The loaded protein concentration was 12.15 mg/g and the immobilized LiP activity was 812.9 U/L. Immobilized LiP had better pH stability. Acid Orange II was used to examine the reusability of immobilized LiP, showing more than 50% of the dye was decolorized at the fifth cycle.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, 99%
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,000
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,900
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~2,700
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Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~5,800
Sigma-Aldrich
Poly(propylene glycol)-block-poly(ethylene glycol)-block-poly(propylene glycol), average Mn ~3,300
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,000
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Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,800
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Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~1,100
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Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~4,400
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~2,900
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~14,600
Sigma-Aldrich
Synperonic® PE P105, surfactant
Sigma-Aldrich
1,3,5-Triisopropylbenzene, 95%
Sigma-Aldrich
Pluronic® F-127, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, ≥98%
Sigma-Aldrich
Synperonic® PE/P84
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, packaged for use in deposition systems, ≥98%
Sigma-Aldrich
Kolliphor® P 188
Sigma-Aldrich
Poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol), average Mn ~8,400
Sigma-Aldrich
Synperonic® F 108, surfactant, non-ionic
Sigma-Aldrich
Kolliphor® P 188, solid
Sigma-Aldrich
Poloxamer 407, purified, non-ionic
Sigma-Aldrich
(3-Aminopropyl)triethoxysilane, ≥98.0%
Sigma-Aldrich
Kolliphor® P 407, oxyethylene 71.5-74.9 %
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Kolliphor® P 188 micro
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
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Sigma-Aldrich
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