The environmental concern pays much attention to the recent cause of the global warming effect. The reduction of the chemical uses is one of many ways to avoid this crucial problem. Herein, the green process for silver nanometallic particle formation and incorporation in gelatin are proposed. By using a novel discharge process in solution named solution plasma, the silver nanometallic particle formation and its incorporation in gelatin could be accomplished in one-batch reactor during discharge by using silver nitrate (AgNO3) solution as the precursor and controlling systematical parameters. The three-dimensional scaffolds of gelatin/silver biocomposite were fabricated using lyophilizer and the water-soluble property of gelatin was improved by irradiation of ultraviolet ray. The well dispersed silver nanoparticles with the mean particle size 10-20 nm in the good texture of gelatin matrix were obtained. The density of micropore in gelatin/silver scaffold was proportional to the gelatin concentration. In addition, thermal stability of prepared samples had no change comparing with pure gelatin, indicating that the incorporation of silver nanoparticles in gelatin matrix did not affect to the nature of gelatin.