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  • A comprehensive behavioral evaluation in the lithium-pilocarpine model in rats: effects of carisbamate administration during status epilepticus.

A comprehensive behavioral evaluation in the lithium-pilocarpine model in rats: effects of carisbamate administration during status epilepticus.

Epilepsia (2013-05-15)
Jean-Baptiste Faure, Gladys Akimana, José E M Carneiro, Brigitte Cosquer, Arielle Ferrandon, Karin Geiger, Estelle Koning, Lorène Penazzi, Jean-Christophe Cassel, Astrid Nehlig
ABSTRACT

Administration of carisbamate during status epilepticus (SE) prevents the occurrence of motor seizures in the lithium-pilocarpine model and leads in a subpopulation of rats to spike-and-wave discharges characteristic of absence epilepsy. Widespread neuroprotection accompanied this change in seizure expression. To assess whether these carisbamate-induced changes affected comorbidity, we used a large battery of behavioral tests in rats that had developed temporal lobe or absence-like seizures. Lithium-pilocarpine or saline was administered to 60 adult rats. Carisbamate (90 mg/kg) or diazepam and saline was given 1 h after SE onset, and repeated 8 h later and twice daily over 6 more days. Rats were video-monitored for 2 months. Subsequently, locomotor activity, anxiety, and various types of memory were assessed. In rats with motor seizures, treated or not with carisbamate, all features of behavior were impaired compared to controls. Rats exhibiting absence-like seizures after carisbamate treatment behaved as controls in all paradigms tested along with widespread neuroprotection. Carisbamate treatment leading to absence-like instead of temporal lobe seizures impressively prevented behavioral comorbidities reported by patients with epilepsy as the most disabling.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pilocarpine hydrochloride, ≥99% (titration), powder
Sigma-Aldrich
Pilocarpine hydrochloride, meets USP testing specifications
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Pilocarpine nitrate salt, ≥98% (HPLC)
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Lithium, ribbon, thickness × W 0.75 mm × 45 mm, 99.9% trace metals basis
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Lithium, ribbon, thickness × W 0.38 mm × 23 mm, 99.9% trace metals basis
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Lithium, ribbon, thickness × W 1.5 mm × 100 mm, 99.9% trace metals basis
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Lithium, wire (in mineral oil), diam. 3.2 mm, 99.9% trace metals basis
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Lithium, ribbon, thickness × W 0.75 mm × 19 mm, 99.9% trace metals basis
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Lithium, granular, 4-10 mesh particle size, high sodium, 99% (metals basis)
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Lithium, rod, diam. 12.7 mm, 99.9% trace metals basis
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Lithium, wire, diam. 3.2 mm, in mineral oil, ≥98%
Sigma-Aldrich
Lithium, granular, 99% trace metals basis
Pilocarpine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Pilocarpine nitrate for system suitability, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Pilocarpine nitrate, meets USP testing specifications