Adenine specific DNA chemical sequencing reaction.

Nucleic acids research (1987-10-12)
B L Iverson, P B Dervan
ABSTRACT

Reaction of DNA with K2PdCl4 at pH 2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCl4 reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium tetrachloropalladate(II), 98%
Sigma-Aldrich
Potassium tetrachloropalladate(II), 98%
Sigma-Aldrich
Potassium tetrachloropalladate(II), 99.99% trace metals basis
Sigma-Aldrich
Sodium tetrachloropalladate(II), ≥99.99% trace metals basis
Sigma-Aldrich
Ammonium tetrachloropalladate(II), 97%
Sigma-Aldrich
Ammonium tetrachloropalladate(II), ≥99.995% trace metals basis