Free-metal ion depletion by "Good's" buffers.

Science (New York, N.Y.) (1983-08-19)
R Nakon, C R Krishnamoorthy

Metal-ion affinity (formation) constants were determined for two "Good's" buffers, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES) and N,N-bis(2-hydroxyethyl)glycine (bicine). The metal chelates formed undergo loss of an internal ligand (alcohol) proton (bicine) and undergo hydrolysis (bicine and TES) and dimerization reactions (TES). Bicine and TES buffer not only hydrogen ions but also metal ions. The metal complexes of "Good's" buffers also buffer hydrogen ions by secondary reactions. The consequences of these reactions are considered in relation to biomedical research.

Product Number
Product Description

BICINE, ≥99% (titration)
BICINE, BioXtra, ≥99% (titration)
Bicine, PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

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