MilliporeSigma
  • Albumin can reverse the release of potassium from human erythrocytes treated with the non-ionic detergent, Brij 58.

Albumin can reverse the release of potassium from human erythrocytes treated with the non-ionic detergent, Brij 58.

Cell biology international (1996-11-01)
P Bogner, D N Wheatley, C Borbély, A Miseta
ABSTRACT

Exchange of erythrocyte intracellular (i/c) K+ for extracellular (e/c) Na+ in human erythrocytes treated with sub-CMC concentrations of the non-ionic detergent Brij 58 can be stopped by reincubation in serum or albumin containing solutions. The progressive equilibration of the K+ contents of detergent-treated human erythrocytes with the incubation medium was reversed by an albumin-mediated withdrawal of detergent molecules from the cell. Re-establishment of near normal [K+] in terms of K+/kg water proceeds in two ways: (i) a metabolism-dependent net accumulation of K+ ions; and (ii) a metabolism-independent shrinkage of erythrocytes, this being the more significant factor.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Brij® S 100, average Mn ~4,670
Sigma-Aldrich
Brij® S20
Sigma-Aldrich
SP Brij® S2 MBAL, main component: diethylene glycol octadecyl ether
Sigma-Aldrich
Brij® S10, average Mn ~711
Sigma-Aldrich
ECO Brij® S100, average Mn ~4,670
Sigma-Aldrich
ECO Brij® S10, average Mn ~711
Sigma-Aldrich
ECO Brij® S20