• Home
  • Search Results
  • Determination of blood sugars by high pressure liquid chromatography with fluorescent detection.

Determination of blood sugars by high pressure liquid chromatography with fluorescent detection.

Journal of pharmaceutical and biomedical analysis (1998-04-21)
Y S Yuh, J L Chen, C H Chiang

In this study, a high pressure liquid chromatography method with fluorescent detector was developed to analyze blood galactose, lactose and glucose simultaneously. Plasma sugars were prepared as fluorescent derivatives to react with FMOC-hydrazine (9-fluorenyl methyl chloroformate). A C18 reversed phase column and a fluorescent detector were used and run in ambient. The resolution index of galactose and glucose derivatives in the analytical method was 1.15. The coefficients of variation of the analysis were less than 7.5%. The concentration of FMOC-hydrazine did not significantly influence the analytical results for determination of the concentration of galactose. However, the ratios of acetonitrile in the mobile phase significantly affected the analysis of the fluorescent derivatives of sugars. The sensitivity of this method for galactose detection was 5 micrograms ml-1, and the required plasma volume for testing was only 25 microliters each. This analytical method was successfully applied to study the pharmacokinetics of galactose in vivo in a rabbit model.

Product Number
Product Description

9-Fluorenylmethyl carbazate, for HPLC derivatization, LiChropur, ≥99.0%

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.