Glucose interference in the pancreolauryl test.

Annals of clinical biochemistry (1998-04-21)
A L Trewick
ABSTRACT

The influence of the glucose concentration in urines being assayed as part of a pancreolauryl test was investigated. Paired patient urines (n = 5) were assayed at 60 degrees and 70 degrees C, before and after spiking to 5% glucose. The influence of assay temperature alone was assessed using glucose-free paired patient urines (n = 10). Aqueous glucose solutions and spiked (5% glucose) normal random urines (n = 5) were assayed to assess the effect of glucose concentration alone. There was no difference in T/K ratios for glucose-free patient samples at 60 degrees, 70 degrees and 80 degrees C. After spiking with glucose T/K ratios were significantly different when assayed at 70 degrees C, but not when assayed at 60 degrees C. Aqueous glucose solutions > or = 0.6% produced a pigment at temperatures > or = 65 degrees C which absorbed at the lambda max of fluorescein. Glucose was found to interfere in the pancreolauryl test. Caution should be exercised when interpreting results from glucosuric samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Fluorescein dilaurate, suitable for fluorescence, ≥97.0% (HPCE)

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