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Simultaneous quantitative analysis of methyl salicylate, ethyl salicylate and salicylic acid from biological fluids using gas chromatography-mass spectrometry.

Journal of chromatography. B, Biomedical sciences and applications (1998-12-01)
T Kakkar, M Mayersohn
ABSTRACT

A gas chromatographic-mass spectrometric (GC-MS) assay was developed for the quantitative analysis of methyl salicylate (MeS), ethyl salicylate (ES) and salicylic acid (SA) from biological fluids. The method was validated from 100-microl rat liver homogenate preparations (5 mg/ml protein) in 70 mM KH2PO4 (pH 7.4) buffer and from 100 microl rat plasma. The samples were extracted with chloroform, derivatized with BSTFA and quantitated by GC-MS in the SIM mode. The standard curves ranged from 31 ng/ml to 800 or 1250 ng/ml. Relative standard deviations and bias were less than 11% in plasma and homogenate for all compounds except SA which evidenced greater variability. The assay was used in preliminary experiments to characterize the pharmacokinetics of MeS in rats.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl salicylate, 99%
Sigma-Aldrich
Ethyl salicylate, ≥99%, FCC, FG
Supelco
Ethyl salicylate, analytical standard

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