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New spectrophotometric procedure for determining cefotaxime based on derivatization with 1,2-naphthoquinone-4-sulphonate into solid-phase extraction cartridges--application to pharmaceutical and urine samples.

Journal of chromatography. B, Biomedical sciences and applications (1998-12-01)
L G Martinez, P Campíns-Falcó, A Sevillano-Cabeza, F Bosch-Reig
ABSTRACT

Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C18) and detected using a UV-visible detection system. Optimum conditions for this new procedure were: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time at 25 degrees C and an NQS concentration of 7.1x10(-3) mol l(-1). The accuracy and the precision of the liquid-solid procedure were tested. The procedure was used to measure cefotaxime in pharmaceutical and urine samples. The results obtained were contrasted with those reported for a HPLC method for urine samples. The generalized H-point standard additions method was used to measure cefotaxime in urine samples.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Naphthoquinone-4-sulfonic acid sodium salt, 97%
Supelco
Sodium 1,2-naphthoquinone-4-sulfonate, for spectrophotometric det. of isonicotic hydrazide, amines and amino acids, ≥97.0% (T)

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