• Home
  • Search Results
  • Mass spectrometric characterization of gentamicin components separated by the new European Pharmacopoeia method.

Mass spectrometric characterization of gentamicin components separated by the new European Pharmacopoeia method.

Journal of pharmaceutical and biomedical analysis (2011-02-15)
B Li, A Van Schepdael, J Hoogmartens, E Adams
ABSTRACT

Liquid chromatography combined with pulsed electrochemical detection (LC-PED) is the method of choice in the European Pharmacopoeia for the determination of gentamicin and its related substances. A recently approved improved LC-PED method, with a reversed-phase C(18) column and a mobile phase consisting of trifluoroacetic acid (TFA), pentafluoropropionic acid (PFPA), sodium hydroxide and acetonitrile, showed better separation and more sensitive detection of the gentamicin components than the previous method using a polymer column. More unknown peaks can be separated from the main components and from each other. As the LC-PED method cannot be directly coupled to a mass spectrometer (MS), the unknown substances were collected after the LC column, desalted and analyzed by MS. The structures of the unknown compounds were deduced based on comparison of their fragmentation patterns with those of reference substances investigated by MS(n) experiments using an electrospray ion trap mass spectrometer. A comparison was also made with an already previously published LC-MS method using a volatile mobile phase.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pentafluoropropionic acid, 97%
Sigma-Aldrich
Sodium pentafluoropropionate, 98%
Sigma-Aldrich
Silver pentafluoropropionate, 98%

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.