• Home
  • Search Results
  • Measurement of the influence of flavonoids on DNA repair kinetics using the comet assay.

Measurement of the influence of flavonoids on DNA repair kinetics using the comet assay.

Food chemistry (2012-09-18)
Catherine Charles, Monia Chemais, Caroline Stévigny, Jacques Dubois, Amandine Nachergael, Pierre Duez
ABSTRACT

The complexity of DNA repair mechanisms infers that xenobiotics, derived from food and medicinal plants, may interfere in the process, activating or inhibiting repair. Different flavonoids were investigated, at the highest non-toxic concentration, for their capacity to modulate DNA repair 12, 24 and 48 h after a non-reactive oxygen species (ROS) treatment involving ethylmethanesulfonate (2mM; 2h). After 12h, DNA fragmentation is substantially increased by quercetin; this effect disappears at subsequent sampling times. At 24h, fragmentation is reduced in the presence of apigenin and slightly increased by sakuranetin. None of the flavonoids tested inhibited repair, which seems complete at 48 h. Ex vivo comet experiments were then performed to assess the excision capabilities of protein extracts obtained from flavonoid-treated cells. Quercetin increases non-specific endonuclease activity, apigenin and epicatechin increase the excision of damages and sakuranetin increases both non-specific and specific enzymatic activities. Combining direct repair and ex vivo experiments yields complementary data that may lead to characterizing mechanisms.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl methanesulfonate, liquid
Supelco
Ethyl methanesulfonate, certified reference material, TraceCERT®

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.