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Ion-pair extraction of histamine from biological fluids and tissues for its determination by high-performance liquid chromatography with fluorescence detection.

Journal of chromatography (1993-04-21)
D Tsikas, R Velásquez, C Toledano, G Brunner
ABSTRACT

A procedure for the selective ion-pair extraction of histamine from various biological fluids and tissues by using the ion-pairing agent bis(2-ethylhexyl)hydrogen-phosphoric acid has been developed. This procedure enabled routine determination of histamine in plasma, urine and tissue samples of humans and rats by reversed-phase high-performance liquid chromatography with fluorescence detection using o-phthaldialdehyde.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Histamine dihydrochloride, ≥99.0% (AT)