β-cyclodextrin cross-linked polymer as solid-phase extraction material coupled with the spectrophotometric method for the analysis of serum albumin.

The β-cyclodextrin cross-linked polymer (β-CDCP) was synthesized and used as solid-phase extraction material to preconcentrate/analysis bovine serum albumin (BSA) coupled with UV-vis spectroscopy. The method based on the complex (BSA-phenylfluorone (PF)) adsorbed on β-CDCP. Adsorption kinetics and various factors of the formation procedure of BSA-PF and its retention on β-CDCP were investigated. The linear range and detection limit (DL) was 20.0-200.0 and 0.03 mg/L, respectively. Moreover, the β-CDCP could be used repeatedly. The proposed method has been applied to analysis serum albumin with satisfactory results.